Laboratory of Experimental and Regenerative Medicine, Department of Medical and Surgical Sciences – University of Foggia
Tecniche utilizzate
Biochemistry
Potentiometry and pH measurements; protein extraction and purification; Bradford and Lowry protein assay; enzymatic activity assay; Western blot; gel electrophoresis; SDS-PAGE;
Immunoprecipitation; ELISA; fluorimetric, spectrophotometric and oxygraphic measurements;
Immunoassays; Immunohistochemistry; Immunocytochemistry
Molecular Biology
Nucleic acids purification and quantification (DNA, RNA) from cells and tissues;
DNA and RNA extraction, purification and PCR-amplification; qRT-PCR; Real Time PCR; gene silencing
