Tecniche utilizzate
Citometria a flusso, colture cellulari, real time PCR, spettrofluorimetria, HPLC-ED, HPLC-UV, ELISA
AINI Laboratory Facilities
AINI Laboratory Facilities
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AINI Laboratory Facilities
Department of Neurology, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DINOGMI)
Tecniche utilizzate
T-cell culture, antigen-specific primary T-cell lines and proliferation assays, NK cell cullture, neural cell culture (astrocytes, microglia, neurons), FACS, electron microscopy, confocal microscopy, ELISA, PCR, Western blot, generation of mouse chimerae -
AINI Laboratory Facilities
Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli “Federico II”
Tecniche utilizzate
Colture cellulari, EAE, FACS sorting, flussi metabolici (Seahorse analyzer), multicolor FACS analysis, biochimica di base -
AINI Laboratory Facilities
Dipartimento di Scienze Neurologiche e del Movimento – Università di Verona
Tecniche utilizzate
Colture cellulari, proteomica, immunoistochimica, microscopia ottica, microscopia confocale, microscopia elettronica -
AINI Laboratory Facilities
I.R.C.C.S. Fondazione Santa Lucia – Unità di Neuroimmunologia, Dipartimento di Neuroscenze Sperimentali
Tecniche utilizzate
Citofluorimetria, ELISA, Western Blot, Colture cellulari, live imaging -
AINI Laboratory Facilities
Istituto di Neurologia Sperimentale, Ospedale San Raffaele – Divisione di Neuroscienze – Immunobiology of Neurological Disorders Unit
Tecniche utilizzate
Molecular and cellular biology of glial/neuronal/immune cells. Functional genomics and bioinformatics. Histology of lymphoid, skeletal and nervous tissue. Experimental mouse models. -
Tecniche utilizzate
Isoelettrofocusing, ELISA, microscopio a fluorescenza, Western blot, elettroforesi verticale, teased nerves, colture cellulari, cell based assays -
Tecniche utilizzate
Cell culture, multi-parametric flow cytometry, gene expression analysis by quantitative real time PCR, protein analysis by electrophoresis, ELISA and cytokine bead array -
AINI Laboratory Facilities
Laboratory of Experimental and Regenerative Medicine, Department of Medical and Surgical Sciences – University of Foggia
Tecniche utilizzate
Biochemistry
Potentiometry and pH measurements; protein extraction and purification; Bradford and Lowry protein assay; enzymatic activity assay; Western blot; gel electrophoresis; SDS-PAGE;
Immunoprecipitation; ELISA; fluorimetric, spectrophotometric and oxygraphic measurements;
Immunoassays; Immunohistochemistry; ImmunocytochemistryMolecular Biology
Nucleic acids purification and quantification (DNA, RNA) from cells and tissues;
DNA and RNA extraction, purification and PCR-amplification; qRT-PCR; Real Time PCR; gene silencing -
AINI Laboratory Facilities
Laboratory of Neuroimmunology, Department of Neuroscience, Drug and Child Health – University of Florence
Tecniche utilizzate
Cell Biology Assays, Molecolar Biology Assays, Confocal Microscopy, Electron Microscopy, Flow Citometry, Luminex Technology, Spectratyping, Immunohistochemistry, Histology, Mice performance tests