Citometria a flusso, colture cellulari, real time PCR, spettrofluorimetria, HPLC-ED, HPLC-UV, ELISA
T-cell culture, antigen-specific primary T-cell lines and proliferation assays, NK cell cullture, neural cell culture (astrocytes, microglia, neurons), FACS, electron microscopy, confocal microscopy, ELISA, PCR, Western blot, generation of mouse chimerae
Colture cellulari, EAE, FACS sorting, flussi metabolici (Seahorse analyzer), multicolor FACS analysis, biochimica di base
Colture cellulari, proteomica, immunoistochimica, microscopia ottica, microscopia confocale, microscopia elettronica
Citofluorimetria, ELISA, Western Blot, Colture cellulari, live imaging
Molecular and cellular biology of glial/neuronal/immune cells. Functional genomics and bioinformatics. Histology of lymphoid, skeletal and nervous tissue. Experimental mouse models.
Isoelettrofocusing, ELISA, microscopio a fluorescenza, Western blot, elettroforesi verticale, teased nerves, colture cellulari, cell based assays
Cell culture, multi-parametric flow cytometry, gene expression analysis by quantitative real time PCR, protein analysis by electrophoresis, ELISA and cytokine bead array
Biochemistry
Potentiometry and pH measurements; protein extraction and purification; Bradford and Lowry protein assay; enzymatic activity assay; Western blot; gel electrophoresis; SDS-PAGE;
Immunoprecipitation; ELISA; fluorimetric, spectrophotometric and oxygraphic measurements;
Immunoassays; Immunohistochemistry; Immunocytochemistry
Molecular Biology
Nucleic acids purification and quantification (DNA, RNA) from cells and tissues;
DNA and RNA extraction, purification and PCR-amplification; qRT-PCR; Real Time PCR; gene silencing
Cell Biology Assays, Molecolar Biology Assays, Confocal Microscopy, Electron Microscopy, Flow Citometry, Luminex Technology, Spectratyping, Immunohistochemistry, Histology, Mice performance tests
Colture cellulari, rt-PCR, microcopia confocale, istologia, patch clamp, microdissezione, analisi di espressione genica, encefalite autoimmune sperimentale, ischemia cerebrale sperimentale, modello di trauma del midollo spinale, modelli transgenici.
Cell biology, nanotechnology, T-cell cloning, cytokine detection, FACS analysis, ELISPOT, polymerase chain reaction, mass spectrometry
Colture cellulari, modelli animali, live imaging, flow cytometry, immunoistochimica.
Tissue culture, molecular biology, EAE, biochemistry, neuropathology, immunology, cell biology
Colture cellulari, immunoscope, MACS, Citofluorimetria, QRT-PCR
Isolamento di tipi cellulari diversi dal circolo periferico (cellule T e B), colture cellulari, estrazione acidi nucleici, real time PCR, digital droplet PCR, genotipizzazione cellulare e virale, deep-sequencing, risonanza magnetica
Cell culture, Cell transplantation, somatin and induced NSCs, Bioinformatics and Computational Biology, next generation sequencing, Confocaland Super-resolution imaging, Electron microscopy (scanning and transmission), Flow cytometry, Fluorescence microscopy,human iPSCs and human iNSCs, Immunohistochemistry, In situ hybridization, In vitro co-cultures, Magnetic resonance imaging (MRI), Microscopy, Non viral delivery technologies (nanoparticles; liposomes), Reverse engineering, RNA deep sequencing, Semi-quantitative real time RT-PCR, siRNA/shRNA, Unbiased stereology, Viral vector technology for cell engineering and ncRNA delivery.